In vitro anti-inflammatory and free radical scavenging properties of Oldenlandia auricularia

Abstract

The in vitro anti-inflammatory and antioxidant properties of extract of Oldenlandia auricularia with 70% aqueous alcohol have been evaluated. The inhibition of nuclear translocation of nuclear factor kappa-light-chain-enhancer of activated B cells in lipopolysaccharide stimulated murine macrophage cells was evaluated. Messenger ribonucleic acid levels of tumor necrosis factor-α and cyclooxygenase-2 were analysed by quantitative real time polymerase chain reaction. O. auricularia (100?mg/L) showed better anti-inflammatory and antioxidant activities when compared to its lower doses, whereas the standard drugs curcumin and diclofenac sodium showed significant anti-inflammatory and antioxidant potential. Treatment at this concentration inhibited the signalling pathway of nuclear factor kappa-light-chain-enhancer of activated B cells and messenger ribonucleic acid expression of tumor necrosis factor- α, and cyclooxygenase-2. Liquid chromatography/quadrupole time-of-flight mass spectrometry revealed the presence of previously reported compounds auricularine, ursolic acid and β-sitosterol. The observed anti-inflammatory property of O. auricularia could be due to the inhibition of nuclear factor kappa-light-chain-enhancer of activated B cells p65 sub unit nuclear translocation and inhibition of its signalling pathway.     

废弃黑木耳菌糠特征及环境影响分析

应用傅里叶变换红外光谱(FTIR)对废弃黑木耳菌糠的分子结构进行表征,结果表明,其含有纤维素、半纤维素、-NH及-COOH等基团。废弃黑木耳菌糠的热稳性能通过热重/微商热重(TG/DTG)进行证实,热分析表明废弃黑木耳菌糠中含有一定的吸附水和结合水,热稳定性良好。表面形貌和元素分布通过扫描电子显微镜(SEM)、电子显微镜(EM)及能谱(EDS)进行解释,废弃黑木耳菌糠表面粗糙而松散,纤维素和菌丝体互相缠绕,其O、C、N、Ca的质量分数为96.24%,Mg、K、S、P、Si、Na、Al的质量分数为3.76%。此外,对废弃黑木耳菌糠的pH、吸水倍率及N、P、K含量进行了测定,其pH为5.14,吸水倍率为466%,N为10.66mg/g,P为2.11mg/g,K为2.61mg/g。并对废弃黑木耳菌糠可能对大气、水及土壤产生的环境影响进行了分析。通过对废弃黑木耳菌糠特征系统的研究和环境影响行为的深入分析,为其综合利用和环境保护提供了理论依据。